GRB7 antibody (Middle Region)

Catalog No. ABIN5518677

This Rabbit Polyclonal antibody specifically detects GRB7 in WB and IHC. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for GRB7 antibody (Middle Region) (ABIN5518677)

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRB7 Antibody

Binding Specificity: AA 124-141, Middle Region

Purpose: Anti-GRB7 Antibody Picoband®

Sequence: ARHVCEMLVQ RAHALSDE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GRB7 Antibody (ABIN5518677). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GRB7, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Tanaka, S., Mori, M., Akiyoshi, T., Tanaka, Y., Mafune, K., Wands, J. R., Sugimachi, K.A novel variant of human Grb7 is associated with invasive esophageal carcinoma.J. Clin. Invest. 102: 821-827, 1998. 2. Vayssiere, B., Zalcman, G., Mahe, Y., Mirey, G., Ligensa, T., Weidner, K. M., Chardin, P., Camonis, J.Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family.FEBS Lett. 467: 91-96, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GRB7 antibody (ABIN5518677)

Zeng, Yang, Hu, Liu, Yang, Ran, Li, Li, Yu: "Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 11296-304, (2016) (PubMed).

Target Details for GRB7

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Alternative Name: GRB7

Background:

Synonyms: Growth factor receptor-bound protein 7,B47,Epidermal growth factor receptor GRB-7,GRB7 adapter protein,GRB7,

Background: GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 thought to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

Sequence Similarities: Belongs to the GRB7/10/14 family.

Molecular Weight: 60 kDa

Gene ID: 2886

UniProt: Q14451

Pathways: EGFR Signaling Pathway, Ribonucleoprotein Complex Subunit Organization