HBV Large S Protein antibody (N-Term)

Catalog No. ABIN5518758

This Rabbit Polyclonal antibody specifically detects HBV Large S Protein in WB and IHC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for HBV Large S Protein antibody (N-Term) (ABIN5518758)

Target: HBV Large S Protein (L-HBsAG) (Hepatitis B Virus Large Surface Protein (L-HBsAG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBV Large S Protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HBV Large S Protein Antibody

Binding Specificity: AA 4-51, N-Term

Purpose: Anti-Hepatitis B Virus Antibody Picoband®

Sequence: WSSKPRQGMG TNLSVPNPLG FFPDHQLDPA FGANSNNPDW DFNPNKDQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Rabbit IgG polyclonal antibody for Large S protein(S) detection. Tested with WB, IHC-P in Human,HBV.
Gene Name: long surface protein, PreS1, L protein, LHBs, large S protein, pre-S1/pre-S2/S regions, L glycoprotein, L-HBsAG, LHBs
Protein Name: Large S protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Hepatitis B Virus.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, HBV
1. Magnius LO, Norder H (1995). "Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene". Intervirology. 38 (1-2): 24-34. 2. Rysgaard, Carolyn D, Morris, Cory S, Drees, Denny, Bebber, Tami, Davis, Scott R, Kulhavy, Jeff, Krasowski, Matthew D (2012). "Positive hepatitis B surface antigen tests due to recent vaccination: a persistent problem". BMC Clinical Pathology. 12 (1): 15. doi:10.1186/1472-6890-12-15. Retrieved11 March 2014.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-HBV Large S Protein antibody (ABIN5518758)

Xiangji, Feng, Qingbao, Weifeng, Xiaoqing, Baihe, Feng, Hongyang, Mengchao: "Knockdown of HBV surface antigen gene expression by a lentiviral microRNA-based system inhibits HBV replication and HCC growth." in: Journal of viral hepatitis, Vol. 18, Issue 9, pp. 653-60, (2011) (PubMed).

Zhang, Wang, Li, Qiao: "Expression of transforming growth factor-alpha and hepatitis B surface antigen in human hepatocellular carcinoma tissues and its significance." in: World journal of gastroenterology, Vol. 10, Issue 6, pp. 830-3, (2004) (PubMed).

Paliaga, Braga: "Passive limitation of adduction after Cüppers's 'Fadenoperation' on medial recti." in: The British journal of ophthalmology, Vol. 73, Issue 8, pp. 633-5, (1989) (PubMed).

Ramsey: "Should medicine today be taught without medical ethics?" in: Connecticut medicine, Vol. 37, Issue 8, pp. 420-1, (1973) (PubMed).

Target Details for HBV Large S Protein

Target: HBV Large S Protein (L-HBsAG) (Hepatitis B Virus Large Surface Protein (L-HBsAG))

Alternative Name: S

Target Type: Viral Protein

Background:

Synonyms: Large S protein ,S ,

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: Hepatitis B virus, abbreviated HBV, is a species of the genus Orthohepadnavirus, which is likewise a part of the Hepadnaviridae family of viruses. This virus causes the disease hepatitis B. It consists of HBsAg, HBcAg (HBeAg is a splice variant), Hepatitis B virus DNA polymerase and HBx. Among these, HBsAg (also known as the Australia antigen) is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection. The viral envelope of an enveloped virus has different surface proteins from the rest of the virus which act as antigens. These antigens are recognized by antibody proteins that bind specifically to one of these surface proteins.

Molecular Weight: 6 kDa

UniProt: D2X4M3