ITGAL antibody (AA 161-349)

Catalog No. ABIN5518766

This anti-ITGAL antibody is a Rabbit Polyclonal antibody detecting ITGAL in FACS, WB, IHC and ICC. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for ITGAL antibody (AA 161-349) (ABIN5518766)

Target: ITGAL (Integrin alpha-L (ITGAL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGAL antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ITGAL Antibody

Binding Specificity: AA 161-349

Purpose: Anti-CD11a/ITGAL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CD11a/ITGAL Antibody Picoband® (ABIN5518766). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. "Entrez Gene: ITGAL integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide)" 2. Marlin, S. D., Morton, C. C., Anderson, D. C., Springer, T. A. LFA-1 immunodeficiency disease: definition of the genetic defect and chromosomal mapping of alpha and beta subunits of the lymphocyte function-associated antigen 1 (LFA-1) by complementation in hybrid cells. J. Exp. Med. 164: 855-867, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ITGAL antibody (ABIN5518766)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Target Details for ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: ITGAL

Background:

Synonyms: Integrin alpha-L,CD11 antigen-like family member A,Leukocyte adhesion glycoprotein LFA-1 alpha chain,LFA-1A,Leukocyte function-associated molecule 1 alpha chain,CD11a,ITGAL,CD11A,

Tissue Specificity: Leukocytes.

Background: CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.

Molecular Weight: 150 kDa

Gene ID: 3683

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex