ALDH1A3 antibody (AA 37-154)

Catalog No. ABIN5518802

This anti-ALDH1A3 antibody is a Rabbit Polyclonal antibody detecting ALDH1A3 in WB, IHC, ICC, IF, FACS and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for ALDH1A3 antibody (AA 37-154) (ABIN5518802)

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ALDH1A3 Antibody

Binding Specificity: AA 37-154

Purpose: Anti-ALDH1A3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ALDH1A3 Antibody Picoband® (ABIN5518802). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Nishimura M, Yoshitsugu H, Naito S, Hiraoka I (2002). "Evaluation of gene induction of drug-metabolizing enzymes and transporters in primary culture of human hepatocytes using high-sensitivity real-time reverse transcription PCR.". Yakugaku Zasshi. 122 (5): 339-61. 2. Rexer BN, Zheng WL, Ong DE (2001). "Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells.". Cancer Res. 61 (19): 7065-70. 3. Yoshida A, Rzhetsky A, Hsu LC, Chang C (1998). "Human aldehyde dehydrogenase gene family.". Eur. J. Biochem. 251 (3): 549-57.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDH1A3

Target: ALDH1A3 (Aldehyde Dehydrogenase 1 Family, Member A3 (ALDH1A3))

Alternative Name: ALDH1A3

Background:

Synonyms: Aldehyde dehydrogenase family 1 member A3,1.2.1.5,Aldehyde dehydrogenase 6,Retinaldehyde dehydrogenase 3,RALDH-3,RalDH3,ALDH1A3,ALDH6,

Tissue Specificity: Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney.

Background: Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Molecular Weight: 56 kDa

Gene ID: 220

UniProt: P47895

Pathways: Retinoic Acid Receptor Signaling Pathway