Annexin IV antibody (Middle Region)

Catalog No. ABIN5518805

This anti-Annexin IV antibody is a Rabbit Polyclonal antibody detecting Annexin IV in WB, IHC and FACS. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for Annexin IV antibody (Middle Region) (ABIN5518805)

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Annexin IV Antibody

Binding Specificity: AA 119-152, Middle Region

Purpose: Anti-Annexin IV/ANXA4 Antibody

Sequence: EEIRRISQTY QQQYGRSLED DIRSDTSFMF QRVL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Annexin IV/ANXA4 Antibody (ABIN5518805). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin IV, different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Annexin IV antibody (ABIN5518805)

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Target Details for Annexin IV

Target: Annexin IV (ANXA4) (Annexin A4 (ANXA4))

Alternative Name: ANXA4

Background:

Synonyms: Annexin A4,35-beta calcimedin,Annexin IV,Annexin-4,Carbohydrate-binding protein p33/p41,Chromobindin-4,Endonexin I,Lipocortin IV,P32.5,PP4-X,Placental anticoagulant protein II,PAP-II,Protein II,ANXA4,ANX4,

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: ANXA4 (Annexin A4), also known as ANX4, is a protein that in humans is encoded by the ANXA4 gene. It belongs to the annexin family of calcium-dependent phospholipid binding proteins. By PCR analysis of somatic cell hybrids and in situ hybridization with a cDNA probe, the human ANXA4 gene is mapped to chromosome 2p13. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. And ANXA4 is almost exclusively expressed in epithelial cells.

Molecular Weight: 36 kDa

Gene ID: 307

UniProt: P09525