IGFBPI antibody (AA 69-259)

Catalog No. ABIN5518851

This Rabbit Polyclonal antibody specifically detects IGFBPI in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for IGFBPI antibody (AA 69-259) (ABIN5518851)

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBPI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IGFBPI Antibody

Binding Specificity: AA 69-259

Purpose: Anti-IGFBP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IGFBP1 Antibody Picoband® (ABIN5518851). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IGFBP1 recombinant protein (Position: A69-N259). Human IGFBP1 shares 67.8% and 69.3% amino acid (aa) sequence identity with mouse and rat IGFBP1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
1. Alitalo, T., Kontula, K., Koistinen, R., Aalto-Setala, K., Julkunen, M., Janne, O. A., Seppala, M., de la Chapelle, A.The gene encoding human low-molecular weight insulin-like growth-factor binding protein (IGF-BP25): regional localization to 7p12-p13 and description of a DNA polymorphism.Hum. Genet. 83: 335-338, 1989. 2. Brinkman, A., Groffen, C. A. H., Kortleve, D. J., Drop, S. L. S.Organization of the gene encoding the insulin-like growth factor binding protein IBP-1.Biochem. Biophys. Res. Commun. 157: 898-907, 1988. 3. Leu, J. I.-J., George, D. L.Hepatic IGFBP1 is a prosurvival factor that binds to BAK, protects the liver from apoptosis, and antagonizes the proapoptotic actions of p53 at mitochondria.Genes Dev. 21: 3095-3109, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IGFBPI

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Alternative Name: IGFBP1

Background:

Synonyms: Insulin-like growth factor-binding protein 1,IBP-1,IGF-binding protein 1,IGFBP-1,Placental protein 12,PP12,IGFBP1,IBP1,

Tissue Specificity: High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.

Background: IGFBP1, Insulin-like growth factor-binding protein 1, also known as placental protein 12 (PP12), is a protein that in humans is encoded by the IGFBP1 gene. The IGFBP1 gene has 4 exons and spans 5.9 kb. And the IGFBP1 gene is localized to 7p13-p12 by in situ hybridization. This gene is a member of the Insulin-like growth factor-binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a type-I thyroglobulin domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molecular Weight: 30 kDa

Gene ID: 3484

UniProt: P08833

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding