NPAS2 antibody (AA 474-578)

Catalog No. ABIN5518939

The Rabbit Polyclonal anti-NPAS2 antibody has been validated for WB. It is suitable to detect NPAS2 in samples from Human, Mouse and Rat.

Quick Overview for NPAS2 antibody (AA 474-578) (ABIN5518939)

Target: NPAS2 (Neuronal PAS Domain Protein 2 (NPAS2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPAS2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NPAS2 Antibody

Binding Specificity: AA 474-578

Purpose: Anti-NPAS2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NPAS2 Antibody Picoband® (ABIN5518939). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human NPAS2 recombinant protein (Position: D474-Q578). Human NPAS2 shares 88.1% amino acid (aa) sequence identity with mouse NPAS2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Hogenesch JB, Chan WK, Jackiw VH, Brown RC, Gu YZ, Pray-Grant M, Perdew GH, Bradfield CA (May 1997). "Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway". J Biol Chem. 272 (13): 8581-93. 2. Zhou YD, Barnard M, Tian H, Li X, Ring HZ, Francke U, Shelton J, Richardson J, Russell DW, McKnight SL (Mar 1997). "Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system". ProcNatlAcadSci USA. 94 (2): 713-8.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NPAS2

Target: NPAS2 (Neuronal PAS Domain Protein 2 (NPAS2))

Alternative Name: NPAS2

Background:

Synonyms: Neuronal PAS domain-containing protein 2,Neuronal PAS2,Basic-helix-loop-helix-PAS protein MOP4,Class E basic helix-loop-helix protein 9,bHLHe9,Member of PAS protein 4,PAS domain-containing protein 4,NPAS2,BHLHE9, MOP4, PASD4,

Background: Neuronal PAS domain-containing protein 2 is a protein that in humans is encoded by the NPAS2 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain.

Molecular Weight: 92 kDa

Gene ID: 4862

UniProt: Q99743

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism