TRAF5 antibody (AA 277-557)

Catalog No. ABIN5518962

This Rabbit Polyclonal antibody specifically detects TRAF5 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRAF5 antibody (AA 277-557) (ABIN5518962)

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TRAF5 Antibody

Binding Specificity: AA 277-557

Purpose: Anti-TRAF5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRAF5 Antibody Picoband® (ABIN5518962). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TRAF5 recombinant protein (Position: K277-L557). Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kraus, Z. J., Nakano, H., Bishop, G. A. TRAF5 is a critical mediator of in vitro signals and in vivo functions of LMP1, the viral oncogenic mimic of CD40. Proc. Nat. Acad. Sci. 106: 17140-17145, 2009. 2. So, T., Salek-Ardakani, S., Nakano, H., Ware, C. F., Croft, M. TNF receptor-associated factor 5 limits the induction of Th2 immune responses. J. Immun. 172: 4292-4297, 2004.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TRAF5

Target: TRAF5 (TNF Receptor-Associated Factor 5 (TRAF5))

Alternative Name: TRAF5

Background:

Synonyms: TNF receptor-associated factor 5,RING finger protein 84,TRAF5,RNF84,

Tissue Specificity: Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.

Background: TNF receptor-associated factor 5, also known as RNF84, is a protein that in humans is encoded by the TRAF5 gene. The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family. Fluorescence in situ hybridization confirmed the regional localization of human TRAF5 to chromosome 1q32.2-q32.3. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. It is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. It seems to be involved in apoptosis.

Molecular Weight: 64 kDa

Gene ID: 7188

UniProt: O00463

Pathways: NF-kappaB Signaling, Apoptosis