GSTT1 antibody (AA 3-231)

Catalog No. ABIN5518998

This Rabbit Polyclonal antibody specifically detects GSTT1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GSTT1 antibody (AA 3-231) (ABIN5518998)

Target: GSTT1 (Glutathione S-Transferase theta 1 (GSTT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GSTT1 Antibody

Binding Specificity: AA 3-231

Purpose: Anti-GSTT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GSTT1 Antibody Picoband® (ABIN5518998). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human GSTT1 recombinant protein (Position: L3-L231).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Pemble, S., Schroeder, K. R., Spencer, S. R., Meyer, D. J., Hallier, E., Bolt, H. M., Ketterer, B., Taylor, J. B.Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem. J. 300: 271-276, 1994.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GSTT1

Target: GSTT1 (Glutathione S-Transferase theta 1 (GSTT1))

Alternative Name: GSTT1

Background:

Synonyms: Glutathione S-transferase theta-1, 2.5.1.18, GST class-theta-1, Glutathione transferase T1-1, GSTT1,

Tissue Specificity: Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

Background: GSTT1 (glutathione S-transferase theta 1), also known as GST class-theta-1, Glutathione transferase T1-1, is an enzyme that in humans is encoded by the GSTT1 gene. It is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human theta-class GST, termed GSTT1 has 239-amino acid GSTT1 protein shares 80 % sequence identity with the rat homolog. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55 % amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

Molecular Weight: 27 kDa

Gene ID: 2952

UniProt: P30711