IL17F antibody (AA 31-163)

Catalog No. ABIN5519013

This Rabbit Polyclonal antibody specifically detects IL17F in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for IL17F antibody (AA 31-163) (ABIN5519013)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL17F Antibody

Binding Specificity: AA 31-163

Purpose: Anti-IL17F Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL17F Antibody Picoband® (ABIN5519013). Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IL17F recombinant protein (Position: R31-Q163).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Starnes, T., Robertson, M. J., Sledge, G., Kelich, S., Nakshatri, H., Broxmeyer, H. E., Hromas, R. Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J. Immun. 167: 4137-4140, 2001. 2. Wang, X., Zhang, Y., Yang, X. O., Nurieva, R. I., Chang, S. H., Ojeda, S. S., Kang, H. S., Schluns, K. S., Gui, J., Jetten, A. M., Dong, C. Transcription of Il17 and Il17f is controlled by conserved noncoding sequence 2. Immunity 36: 23-31, 2012.

Comment:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL17F

Background:

Synonyms: Interleukin-17F, IL-17F, Cytokine ML-1, IL17F,

Tissue Specificity: Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.

Background: Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.

Molecular Weight: 18 kDa, 30 kDa

Gene ID: 112744

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity