SUPT5H antibody (AA 395-424)

Catalog No. ABIN5531040

The Rabbit Polyclonal anti-SUPT5H antibody has been validated for WB. It is suitable to detect SUPT5H in samples from Human.

Quick Overview for SUPT5H antibody (AA 395-424) (ABIN5531040)

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT5H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SUPT5H Antibody

Binding Specificity: AA 395-424

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SUPT5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the Central region of human SUPT5H.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SUPT5H

Target: SUPT5H (Transcription Elongation Factor SPT5 (SUPT5H))

Alternative Name: SUPT5H

Background: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Molecular Weight: 121 kDa

Gene ID: 6829

UniProt: O00267