CHMP4B antibody (N-Term)

Catalog No. ABIN5536006

This anti-CHMP4B antibody is a Rabbit Polyclonal antibody detecting CHMP4B in WB, FACS and IHC (p). Suitable for Human.

Quick Overview for CHMP4B antibody (N-Term) (ABIN5536006)

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP4B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHMP4B Antibody

Binding Specificity: AA 51-79, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CHMP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human CHMP4B.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CHMP4B

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Alternative Name: CHMP4B

Background: CHMP4B is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.

Molecular Weight: 25 kDa

Gene ID: 128866

UniProt: Q9H444