ROBO3 antibody (Internal Region)

Catalog No. ABIN5539585

Product Details anti-ROBO3 Antibody

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Purpose: ROBO3 (internal)

Sequence: RKDGARLKEE EGR

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml. This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF): Denk et al (2010) PMID: 20298552.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3 (ROBO3 Products)

Background: ROBO3, roundabout, axon guidance receptor, homolog 3 (Drosophila) , HGNC:13433, FLJ21044, HGPPS, RBIG1, RIG1 , retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3

Gene ID: 64221

NCBI Accession: NP_071765