ROBO3 antibody

Catalog No. ABIN7261161

Product Details anti-ROBO3 Antibody

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO3 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human ROBO3 (NP_071765.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3 (ROBO3 Products)

Synonyms: HGPPS antibody, HGPS antibody, RBIG1 antibody, RIG1 antibody, CG14345 antibody, CT17158 antibody, Robo-3 antibody, Robo3 antibody, dRobo-3 antibody, DmelCG5423 antibody, CG5423 antibody, ROBO3 antibody, robo3 antibody, DKFZp468G0223 antibody, Rbig1 antibody, Rig-1 antibody, Rig1 antibody, Robo3a antibody, Robo3b antibody, cb503 antibody, roundabout guidance receptor 3 antibody, roundabout 3 antibody, robo3 antibody, roundabout guidance receptor 3 L homeolog antibody, roundabout, axon guidance receptor, homolog 3 (Drosophila) antibody, ROBO3 antibody, robo3 antibody, Robo3 antibody, LOC658818 antibody, robo3.L antibody

Background: This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated.

Molecular Weight:

Observed_MW: 200 kDa

Calculated_MW: 110 kDa/148 kDa

Gene ID: 64221

UniProt: Q96MS0