CD3 epsilon antibody (Internal Region)
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- Target See all CD3 epsilon (CD3E) Antibodies
- CD3 epsilon (CD3E)
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CD3 epsilon antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- CD3E (cytoplasmic)
- Sequence
- RKGQRDLYSG LNQR
- Specificity
- This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
- Cross-Reactivity
- Human, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product CD3E Primary Antibody
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E Reactivity: Human, Mouse, Rat WB, IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E Reactivity: Human, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig, Chicken FACS, WB, IF Host: Mouse Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E Reactivity: Human, Mouse, Rat FACS, IF, Func Host: Mouse Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E Reactivity: Human FACS, WB, IHC, ELISA, Coat, StM Host: Mouse Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E Reactivity: Human WB, IHC, IF, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Background
- CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro
- Gene ID
- 916, 315609
- NCBI Accession
- NP_000724
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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