KRT20 antibody (Internal Region)

Catalog No. ABIN5539706

This Goat Polyclonal antibody specifically detects KRT20 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for KRT20 antibody (Internal Region) (ABIN5539706)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-KRT20 Antibody

Binding Specificity: Internal Region

Purpose: Cytokeratin 20

Sequence: ETNAPRAGRD YS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 5 μg/mL.

Western Blot: Approx 49 kDa band observed in Human Colon and Ileum lysates (calculated MW of 48.5 kDa according to NP_061883.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20

Background: KRT20, keratin 20, CK-20, CK20, K20, KRT21, MGC35423, OTTHUMP00000164518, cytokeratin 20, cytokeratin-20, keratin, type I cytoskeletal 20, keratin-20, protein IT

Gene ID: 54474

NCBI Accession: NP_061883