UBE2I antibody (N-Term) (Biotin)

Catalog No. ABIN5539759

This anti-UBE2I antibody is a Goat Polyclonal antibody detecting UBE2I in WB, IHC, ELISA and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for UBE2I antibody (N-Term) (Biotin) (ABIN5539759)

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2I antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-UBE2I Antibody (Biotin)

Binding Specificity: N-Term

Purpose: UBE2I, Biotinylated

Sequence: SGIALSRLAQ ERKC.

Specificity: Reported variants represent identical protein: NP_919237.1, NP_003336.1, NP_919236.1, NP_919235.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: .

Western Blot: Approx 17 kDa band observed in Human Kidney lysates (calculated MW of 18.0 kDa according to Human NP_003336.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I

Background: UBE2I, ubiquitin-conjugating enzyme E2I, C358B7.1, P18, UBC9, SUMO-1-protein ligase, SUMO-protein ligase, ubiquitin carrier protein 9, ubiquitin carrier protein I, ubiquitin conjugating enzyme 9, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), ubi

Gene ID: 7329, 22196, 25573

NCBI Accession: NP_003336

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway