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CD3D antibody (Internal Region)

This anti-CD3D antibody is a Goat Polyclonal antibody detecting CD3D in WB and ELISA. Suitable for Human.
Catalog No. ABIN5539780

Quick Overview for CD3D antibody (Internal Region) (ABIN5539780)

Target

See all CD3D Antibodies
CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity

  • 58
  • 31
  • 20
  • 7
  • 6
  • 3
  • 2
  • 2
Human

Host

  • 87
  • 28
  • 2
  • 2
  • 1
Goat

Clonality

  • 82
  • 38
Polyclonal

Conjugate

  • 64
  • 23
  • 15
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This CD3D antibody is un-conjugated

Application

  • 86
  • 59
  • 44
  • 28
  • 23
  • 19
  • 12
  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA

Grade

Verified
  • Binding Specificity

    • 13
    • 9
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Purpose

    CD3D (isoform A)

    Sequence

    HETGRLSGAA DTQ

    Specificity

    This antibody is expected to recognize reported isoform A (NP_000723.1) only.

    Cross-Reactivity

    Human

    Purification

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    Isotype

    IgG
  • Application Notes

    Western Blot: Approx 20 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 18.9 kDa according to NP_000723.1). Recommended concentration: 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Minimize freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target

    CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

    Alternative Name

    CD3D

    Background

    CD3D, CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, IMD19, T3D, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), OKT3, delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain

    Gene ID

    915

    NCBI Accession

    NP_000723

    Pathways

    TCR Signaling, CXCR4-mediated Signaling Events
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