PDCD4 antibody (AA 210-221)

Catalog No. ABIN5539877

This Goat Polyclonal antibody specifically detects PDCD4 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PDCD4 antibody (AA 210-221) (ABIN5539877)

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PDCD4 Antibody

Binding Specificity: AA 210-221

Purpose: PDCD4 (aa210-221)

Sequence: EGKASHREMT SK

Specificity: This antibody is expected to recognize all reported isoforms (NP_055271.2, NP_663314.1, NP_001186421.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 50 kDa band observed in lysates of cell line HeLa (calculated MW of 50.2 kDa according to NP_001186421.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4

Background: PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), H731, MGC33046, MGC33047, OTTHUMP00000020483, neoplastic transformation inhibitor protein, nuclear antigen H731, programmed cell death protein 4, protein 197/15a

Gene ID: 27250, 18569, 64031

NCBI Accession: NP_055271, NP_663314, NP_001186421