Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN5539990

This anti-Intestinal Alkaline Phosphatase antibody is a Goat Polyclonal antibody detecting Intestinal Alkaline Phosphatase in WB, IP, EIA and DB. Suitable for Cow.

Quick Overview for Intestinal Alkaline Phosphatase antibody (ABIN5539990)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Specificity: This antibody detects bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Goat Serum as well as purified and partially purified alkaline phosphatase [calf intestine]. Species: Bovine.

Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

Immunogen: Alkaline phosphatase from calf intestine

Isotype: IgG

Application Details

Application Notes: ELISA : 1/4000-1/20000. Western Blot : 1/500-1/2000. Immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Restrictions: For Research Use only

Handling

Buffer: 0.01M Sodium phosphate, 0.14M Sodium chloride, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: alkaline phosphatase,alpi,iap

Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

UniProt: P19111