GAP43 antibody (C-Term)

Catalog No. ABIN5540033

This Chicken Polyclonal antibody specifically detects GAP43 in WB, IF, ICC and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for GAP43 antibody (C-Term) (ABIN5540033)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GAP43 Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~43k GAP-43 protein.

Purification: Affinity Chromatography

Immunogen: Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Quality Control: Quality assurance analysis was performed using Immunohistochemistry (at 1/1000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/1000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemstry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells and 1/2000-1/5000 for western blots.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: gap43

Background: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

UniProt: P17677