LYZ antibody

Catalog No. ABIN5540464

This anti-LYZ antibody is a Rabbit Polyclonal antibody detecting LYZ in WB, ICC, IF, IHC (p), EIA and DB. Suitable for Human.

Quick Overview for LYZ antibody (ABIN5540464)

Target: LYZ (Lysozyme (LYZ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-LYZ Antibody

Specificity: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. Cross-reactivity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography

Immunogen: Highly purified lysozyme is isolated from pooled milk.Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunohistochemistry on Paraffin Sections: 1/400.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2 Preservatives: None Stabilizers:

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: lysozyme c

Background: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low in milk the concentration is reported to be between 5 and 220 mg/mL. Also tears contain a considerable amount of lysozyme.

UniProt: P00698