ICT1 antibody (AA 30-206)

Catalog No. ABIN5540729

This Mouse Monoclonal antibody specifically detects ICT1 in WB, FACS and EIA. It exhibits reactivity toward Human.

Quick Overview for ICT1 antibody (AA 30-206) (ABIN5540729)

Target: ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICT1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: AT1E9

Product Details anti-ICT1 Antibody

Binding Specificity: AA 30-206

Specificity: This antibody detects ICT1 at aa30-206.

Purification: Protein-A affinity chromatography

Immunogen: Recombinant human ICT1 (30-206aa) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: Recommended starting dilution is 1:1000. Flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ICT1

Target: ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))

Alternative Name: ict1,ds1

Background: Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.

UniProt: Q14197