Actin antibody

Catalog No. ABIN5540756

This Chicken Polyclonal antibody specifically detects Actin in WB. It exhibits reactivity toward Rabbit.

Quick Overview for Actin antibody (ABIN5540756)

Target: Actin

Reactivity: Rabbit

Host: Chicken

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Actin Antibody

Purification: Affinity Chromatography

Immunogen: Chickens were immunized with actin purified from Rabbit muscle. After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 mug/ml of affinity purified anti-Actin IgY, and then filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 μg/mL) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent. Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary. Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Actin

Target: Actin