beta Actin antibody (N-Term)

Catalog No. ABIN5540759

This Mouse Monoclonal antibody specifically detects beta Actin in WB, IF, IHC (p), ICC, FACS, IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Rabbit, Pig and Zebrafish (Danio rerio).

Quick Overview for beta Actin antibody (N-Term) (ABIN5540759)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Pig, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 4C2

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Sequence: Ac-DDDIAALVV-COOH

Specificity: This 4C2 Monoclonal antibody is highly specific for β-Cytoplasmic Actin. The antibody does not cross react with other Actin isoforms.

Purification: Purified

Immunogen: Peptide comprising the N-terminal nonapeptide of beta-Cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue

Isotype: IgG1

Application Details

Application Notes: ELISA. Flow cytometry. Western blot. Immunocytochemistry. Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin-embedded sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: actin beta,actb

Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction