LALBA antibody (HRP)

Catalog No. ABIN5541329

This Rabbit Polyclonal antibody specifically detects LALBA in WB, ICC, IHC (p), IF, EIA and DB. It exhibits reactivity toward Human.

Quick Overview for LALBA antibody (HRP) (ABIN5541329)

Target: LALBA (Lactalbumin, alpha- (LALBA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LALBA antibody is conjugated to HRP

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB)

Product Details anti-LALBA Antibody (HRP)

Specificity: The defined antibody specificity is directed to alpha-lactalbumin as tested against human milk. Its presence can also be demonstrated in udder tissues. When tested in immunoelectrophoresis and double radial immunodiffusion against serum no reaction is obtained. Cross-reactivity: Homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has not been tested for cross-reactivity. Species: Human.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Highly purified alpha-lactalbumin isolated from Human milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Dot blot. Immunoblotting. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of alpha lactalbumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure alpha lactalbumin in serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions Histochemical and Cytochemical Use: 1/100-1/250 ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LALBA

Target: LALBA (Lactalbumin, alpha- (LALBA))

Alternative Name: lalba

Background: Alpha-lactalbumin is one of the major proteins in the alpha region after immunoelectrophoresis of human milk. It has been implicated as a component of the enzyme lactose synthetase which catalyzes the formation of lactose from uridine diphosphate galactose and glucose.

UniProt: P00709