This product detects porcine Mutarotase / Aldose 1-epimerase. Cross reactivity against Mutarotase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified porcine Mutarotase. Species: Porcine (Pig).
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western blot : 1/500-1/5,000. Immunoprecipitation : 1/100. ELISA : 1/5,000-1/20,000. This product has been assayed against 1.0 μg of Mutarotase [porcine kidney] in a standard sandwich ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/1,500 of the reconstitution concentration is suggested.
Restrictions
For Research Use only
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.