The Goat Polyclonal anti-Invertase antibody has been validated for WB and EIA. It is suitable to detect Invertase in samples from Candida sp..
Catalog No. ABIN5541873
Quick Overview for Invertase antibody (HRP) (ABIN5541873)
Target
Invertase
Reactivity
Candida sp.
Host
Goat
Clonality
Polyclonal
Conjugate
This Invertase antibody is conjugated to HRP
Application
Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificity
This antibody detects Invertase [Candida]. Cross reactivity against Invertase from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Invertase [Candida].
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Western blot : 1/500-1/2,500. ELISA : 1/1,000-1/5,000. This product has been assayed against 1.0 μg of Invertase [Candida] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/15,000 of the reconstitution concentration is suggested.
Restrictions
For Research Use only
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
Invertase
Background
Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.