CD81 antibody

Catalog No. ABIN5541933

The Rat Monoclonal anti-CD81 antibody has been validated for FACS and EIA. It is suitable to detect CD81 in samples from Human.

Quick Overview for CD81 antibody (ABIN5541933)

Target: CD81

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: QV-6A8-S3

Product Details anti-CD81 Antibody

Specificity: Recognizes Human CD81. Other species not tested. Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.

Purification: Affinity Chromatography on Protein G

Immunogen: cDNA encoding Human CD81

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 1.2 μg/10 6 cells. ELISA: 1/200-1/400. Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD81

Target: CD81

Alternative Name: cd81,tapa1

Background: CD81 (TAPA-1) belongs to the transmembrane 4 superfamily (tetraspanin family). CD81 is a widely expressed cell-surface protein that is characterized by the presence of four transmembranic domains, short N and C termini, a small extracellular loop (SEL) and a large extra-cellular loop (LEL) (1). It is expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin and plays a role in the regulation of cell development, cell-growth and signal transduction. CD81 plays a critical role in Hepatitis C Infection and is involved in HCV entry due to its ability to interact with virus' E1/E2 glycoproteins (2,3). CD81 is an essential HCV host factor as silencing of CD81 expression by CD81-specific monoclonal antibodies in hepatoma cells inhibits HCV entry (4).

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C