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Methylated Lysine antibody (Biotin)

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB and ELISA. Suitable for Various Species.
Rockland
Catalog No. ABIN5596699
Supplier Product No.: 600-406-940

Quick Overview for Methylated Lysine antibody (Biotin) (ABIN5596699)

Target

Methylated Lysine

Reactivity

  • 15
  • 15
  • 4
Various Species

Host

  • 32
  • 4
Rabbit

Clonality

  • 32
  • 4
Polyclonal

Conjugate

  • 9
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 36
  • 19
  • 14
  • 13
  • 13
  • 9
  • 6
  • 4
  • 3
  • 2
  • 2
Western Blotting (WB), ELISA
  • Supplier Product No.

    600-406-940

    Supplier

    Rockland

    Purpose

    Lysine Methylated Antibody Biotin Conjugated

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

    Characteristics

    Synonyms: Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody

    Purification

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.

    Sterility

    Sterile filtered

    Immunogen

    Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.

    Immunogen Type: Other

    Isotype

    IgG
  • Application Notes

    Application Note: This affinity-purified antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with methylated lysine residues.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:5,000

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: None

    Preservative

    Without preservative

    Storage

    -20 °C

    Storage Comment

    Store vial at -20° C or below prior to opening.  Dilute only prior to immediate use.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    Expiry Date

    12 months
  • Target

    Methylated Lysine

    Alternative Name

    Lysine Methylated

    Target Type

    Chemical

    Background

    Background: Anti-Lysine Methylated (MeK) Antibody Biotin Conjugated is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.
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