Methylated Lysine antibody (HRP)

Catalog No. ABIN5596700

Supplier Product No.: 600-403-940

This Rabbit Polyclonal antibody specifically detects in WB and ELISA. It exhibits reactivity toward Various Species.

Quick Overview for Methylated Lysine antibody (HRP) (ABIN5596700)

Target: Methylated Lysine

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Product Details

Supplier Product No.: 600-403-940

Supplier: Rockland

Purpose: Lysine Methylated Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.

Characteristics: Synonyms: Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody is suitable for use in ELISA and western blotting.  The antibody reacts specifically with methylated lysine residues.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Methylated Lysine

Alternative Name: Lysine Methylated

Target Type: Chemical

Background: Background: Anti-Lysine Methylated (MeK) Antibody HRP Conjugated is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.