beta Actin antibody

Catalog No. ABIN559692

This anti-beta Actin antibody is a Mouse Monoclonal antibody detecting beta Actin in WB, ELISA, DB and ISt. Suitable for Human, Mouse, Rat, Chicken and Rabbit. This Primary Antibody has been cited in 3+ publications.

Quick Overview for beta Actin antibody (ABIN559692)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Clone: BA3R

Product Details anti-beta Actin Antibody

Specificity: Recognizes native and denatured forms of Beta-Actin (∼42kDa). Beta-Actin from human, mouse, rat, rabbit, and chicken / Beta-Actin from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Beta-Actin N-terminal peptide-KLH conjugates

Isotype: IgG2b

Application Details

Application Notes: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-beta Actin antibody (ABIN559692)

Steponaitis, Kazlauskas, Skiriute, Valiulyte, Skauminas, Tamasauskas, Vaitkiene: "Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma." in: Oncology letters, Vol. 12, Issue 5, pp. 3305-3311, (2016) (PubMed).

Vaitkienė, Skiriutė, Steponaitis, Skauminas, Tamašauskas, Kazlauskas: "High level of Sema3C is associated with glioma malignancy." in: Diagnostic pathology, Vol. 10, pp. 58, (2015) (PubMed).

Skiriutė, Steponaitis, Vaitkienė, Mikučiūnas, Skauminas, Tamašauskas, Kazlauskas: "Glioma Malignancy-Dependent NDRG2 Gene Methylation and Downregulation Correlates with Poor Patient Outcome." in: Journal of Cancer, Vol. 5, Issue 6, pp. 446-56, (2014) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Beta-Actin

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction