MDH1 antibody (HRP)

Catalog No. ABIN5596953

Supplier Product No.: 200-603-145-0100

The Sheep Polyclonal anti-MDH1 antibody has been validated for WB, ELISA, IP and IHC. It is suitable to detect MDH1 in samples from Pig.

Quick Overview for MDH1 antibody (HRP) (ABIN5596953)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This MDH1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-MDH1 Antibody (HRP)

Supplier Product No.: 200-603-145-0100

Supplier: Rockland

Purpose: Malate Dehydrogenase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Characteristics: Synonyms: sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase

Purification: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:250

Application Note: Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:50

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDH1

Background: Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.

Gene ID: 396894

UniProt: P11708