Protein A antibody (Texas Red (TR))

Catalog No. ABIN5596971

Supplier Product No.: 200-109-077

The Goat Polyclonal anti-Protein A antibody has been validated for FACS and FM. It is suitable to detect Protein A in samples from Staphylococcus aureus.

Quick Overview for Protein A antibody (Texas Red (TR)) (ABIN5596971)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to Texas Red (TR)

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-Protein A Antibody (Texas Red (TR))

Supplier Product No.: 200-109-077

Supplier: Rockland

Purpose: Protein A Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.

Characteristics: Synonyms: goat anti-Protein A Antibody, Texas Red™ Conjugated goat anti-Protein A Antibody, Protein A Texas Red™, Texas Red™, Protein A Texas Red™ Antibody

Purification: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 1.89

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Protein A Texas Red Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Background: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.