ATF4 antibody (AA 171-270)

Catalog No. ABIN559979

The Mouse Monoclonal anti-ATF4 antibody has been validated for WB, ELISA and IF. It is suitable to detect ATF4 in samples from Human. There are 5+ publications available.

Quick Overview for ATF4 antibody (AA 171-270) (ABIN559979)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 2B3

Product Details anti-ATF4 Antibody

Binding Specificity: AA 171-270

Purpose: Mouse monoclonal antibody raised against a partial recombinant ATF4.

Sequence: SSTPDHSFSL ELGSEVDITE GDRKPDYTAY VAMIPQCIKE EDTPSDNDSG ICMSPESYLG SPQHSPSTRG SPNRSLPSPG VLCGSARPKP YDPPGEKMVA

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ATF4 (NP_001666.2, 171 a.a. ~ 270 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ATF4 antibody (ABIN559979)

Ghemrawi, Pooya, Lorentz, Gauchotte, Arnold, Gueant, Battaglia-Hsu: "Decreased vitamin B12 availability induces ER stress through impaired SIRT1-deacetylation of HSF1." in: Cell death & disease, Vol. 4, pp. e553, (2013) (PubMed).

Wang, Wang, Zhang, Qu, Liu: "A protective role of heme-regulated eIF2? kinase in cadmium-induced toxicity in erythroid cells." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 62, pp. 880-91, (2013) (PubMed).

Cole, Kuhn, Green-Mitchell, Leone, Raab, Nadler, Chakrabarti: "12/15-Lipoxygenase signaling in the endoplasmic reticulum stress response." in: American journal of physiology. Endocrinology and metabolism, Vol. 302, Issue 6, pp. E654-65, (2012) (PubMed).

Suragani, Zachariah, Velazquez, Liu, Sun, Townes, Chen: "Heme-regulated eIF2? kinase activated Atf4 signaling pathway in oxidative stress and erythropoiesis." in: Blood, Vol. 119, Issue 22, pp. 5276-84, (2012) (PubMed).

Meister, Frey, Lang, Gaipl, Schett, Schlötzer-Schrehardt, Voll: "Calcium channel blocker verapamil enhances endoplasmic reticulum stress and cell death induced by proteasome inhibition in myeloma cells." in: Neoplasia (New York, N.Y.), Vol. 12, Issue 7, pp. 550-61, (2010) (PubMed).

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4

Background: Full Gene Name: activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms: CREB-2,CREB2,TAXREB67,TXREB

Gene ID: 468

NCBI Accession: NM_001675

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response