CRX antibody (AA 1-95)

Catalog No. ABIN560473

This Mouse Monoclonal antibody specifically detects CRX in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 10+ publications.

Quick Overview for CRX antibody (AA 1-95) (ABIN560473)

Target: CRX (Cone-Rod Homeobox (CRX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4G11

Product Details anti-CRX Antibody

Binding Specificity: AA 1-95

Purpose: Mouse monoclonal antibody raised against a partial recombinant CRX.

Sequence: MMAYMNPGPH YSVNALALSG PSVDLMHQAV PYPSAPRKQR RERTTFTRSQ LEELEALFAK TQYPDVYARE EVALKINLPE SRVQVWFKNR RAKCR

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CRX (NP_000545, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CRX antibody (ABIN560473)

Khan, Walters, Li, Thomas, Miller, Zhang, Sciallis, Liu, Dlouhy, Fort, Archer, Demirci, Dou, Rao: "Characterization and pharmacologic targeting of EZH2, a fetal retinal protein and epigenetic regulator, in human retinoblastoma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 11, pp. 1278-90, (2015) (PubMed).

Reichman, Goureau: "Production of Retinal Cells from Confluent Human iPS Cells." in: Methods in molecular biology (Clifton, N.J.), Vol. 1357, pp. 339-51, (2015) (PubMed).

Tran, Zhang, Zhang, Huecker, Hennig, Chen: "Mechanistically distinct mouse models for CRX-associated retinopathy." in: PLoS genetics, Vol. 10, Issue 2, pp. e1004111, (2014) (PubMed).

Jayaram, Jones, Eastlake, Cottrill, Becker, Wiseman, Khaw, Limb: "Transplantation of photoreceptors derived from human Muller glia restore rod function in the P23H rat." in: Stem cells translational medicine, Vol. 3, Issue 3, pp. 323-33, (2014) (PubMed).

Reichman, Terray, Slembrouck, Nanteau, Orieux, Habeler, Nandrot, Sahel, Monville, Goureau: "From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 23, pp. 8518-23, (2014) (PubMed).

Xie, Zhang, Hashimoto, Tien, Chen, Ge, Yang: "Differentiation of retinal ganglion cells and photoreceptor precursors from mouse induced pluripotent stem cells carrying an Atoh7/Math5 lineage reporter." in: PLoS ONE, Vol. 9, Issue 11, pp. e112175, (2014) (PubMed).

Carr, Vugler, Yu, Semo, Coffey, Moss, Greenwood: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, (2011) (PubMed).

Sakagami, Gan, Yang: "Distinct effects of Hedgehog signaling on neuronal fate specification and cell cycle progression in the embryonic mouse retina." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 21, pp. 6932-44, (2009) (PubMed).

Esumi, Kachi, Hackler, Masuda, Yang, Campochiaro, Zack: "BEST1 expression in the retinal pigment epithelium is modulated by OTX family members." in: Human molecular genetics, Vol. 18, Issue 1, pp. 128-41, (2008) (PubMed).

Peng, Chen: "Crx activates opsin transcription by recruiting HAT-containing co-activators and promoting histone acetylation." in: Human molecular genetics, Vol. 16, Issue 20, pp. 2433-52, (2007) (PubMed).

Target Details for CRX

Target: CRX (Cone-Rod Homeobox (CRX))

Alternative Name: CRX

Background: Full Gene Name: cone-rod homeobox
Synonyms: CORD2,CRD,LCA7,OTX3

Gene ID: 1406

NCBI Accession: NM_000554