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Histone 3 antibody (C-Term)

Name: Histone 3 antibody (C-Term)
SKU: ABIN5608733
Price: 472.31 USD
Availability: InStock

This Mouse Monoclonal antibody specifically detects Histone 3 in WB and IF (cc). It exhibits reactivity toward Human, Tomato, Poplar (Populus) and Zea mays.

Western blot was performed on whole cell extracts from HeLa cells (25 μg, lane 1) and recombinant histone H2A, H2B, H3 and H4 (1ug, lane 2, 3, 4 and 5, respectively) using the H3pan (1B2) Monoclonal Antibody. (Histone 3 antibody (C-Term))

HeLa cells stained with the H3pan Monoclonal Antibody and with DAPI. (Histone 3 antibody (C-Term))

IF (cc)

Western blot was performed on whole cell extracts (30μg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using H3pan Monoclonal Antibody at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation and exposure. (Histone 3 antibody (C-Term))

3 images

Catalog No. ABIN5608733

Quick Overview for Histone 3 antibody (C-Term) (ABIN5608733)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1344
909
811
41
39
39
34
33
26
23
21
21
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human, Tomato, Poplar (Populus), Zea mays

Host

Mouse

Clonality

Monoclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1028
418
374
323
253
202
187
187
163
159
148
127
115
58
48
48
35
22
16
14
7
7
6
4
3
1
1
1
1

Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone

1B2

Discover our top product H3 Primary Antibody

Binding Specificity
52

50

48

44

39

37

37

36

35

35

34

33

32

31

31

30

23

23

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22

22

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11

10

10

9

9

9

8

8

8
C-Term

Cross-Reactivity

Human

Cross-Reactivity (Details)

maize, tomato, poplar

Purification

Protein A purified monoclonal antibody in PBS containing 0.05% azide.

Immunogen

Monoclonal antibody raised in mouse against histone H3, using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein.

Isotype

IgG3
anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 Reactivity: Human WB, IF, IHC, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Concentration

0.5 μg/μL

Buffer

PBS with 0.05 % sodium azide.

Storage

-20 °C

Storage Comment

Store at -20°C for 12 months.
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

H3

Background

Optional[synonyms]: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

8350

UniProt

P68431