Alkaline Phosphatase antibody (AA 403-433)

Catalog No. ABIN5647037

This anti-Alkaline Phosphatase antibody is a Rabbit Polyclonal antibody detecting Alkaline Phosphatase in WB and IHC (p). Suitable for Human.

Quick Overview for Alkaline Phosphatase antibody (AA 403-433) (ABIN5647037)

Target: Alkaline Phosphatase (ALP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Alkaline Phosphatase Antibody

Binding Specificity: AA 403-433

Purification: Antigen affinity purified

Immunogen: Amino acids 403-433 (KARDRKAYTVLLYGNGPGYVLKDGARPDVTE-human) were used as the immunogen for the PLAP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLAP antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PLAP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline phosphatase

Background: Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

UniProt: P05187