SAV1 antibody (AA 300-383)

Catalog No. ABIN566210

The Mouse Monoclonal anti-SAV1 antibody has been validated for WB, ELISA, IF and IP. It is suitable to detect SAV1 in samples from Human. There are 6+ publications available.

Quick Overview for SAV1 antibody (AA 300-383) (ABIN566210)

Target: SAV1 (Salvador Homolog 1 (SAV1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SAV1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 3B2

Product Details anti-SAV1 Antibody

Binding Specificity: AA 300-383

Purpose: Mouse monoclonal antibody raised against a partial recombinant SAV1.

Sequence: HTAEIPDWLQ VYARAPVKYD HILKWELFQL ADLDTYQGML KLLFMKELEQ IVKMYEAYRQ ALLTELENRK QRQQWYAQQH GKNF

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SAV1 (NP_068590, 300 a.a. ~ 383 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SAV1 antibody (ABIN566210)

Kai, Tsukamoto, Hijiya, Tokunaga, Nakada, Uchida, Daa, Iha, Takahashi, Nomura, Sato, Mimata, Ikawa, Seto, Matsuura, Moriyama: "Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway." in: The Journal of pathology, Vol. 239, Issue 1, pp. 97-108, (2016) (PubMed).

Schütte, Bisht, Heukamp, Kebschull, Florin, Haarmann, Hoffmann, Bendas, Buettner, Brossart, Feldmann: "Hippo signaling mediates proliferation, invasiveness, and metastatic potential of clear cell renal cell carcinoma." in: Translational oncology, Vol. 7, Issue 2, pp. 309-21, (2014) (PubMed).

Park, Kim, Won, Jeon, Oh, Lee, Kim, Lee: "Mammalian ste20-like kinase and SAV1 promote 3T3-L1 adipocyte differentiation by activation of PPAR?." in: PLoS ONE, Vol. 7, Issue 1, pp. e30983, (2012) (PubMed).

Li, Luo, Li, Wang, Xiao, Tao, Gong, Hu: "Screening of binding proteins that interact with human Salvador 1 in a human fetal liver cDNA library by the yeast two-hybrid system." in: Molecular biology reports, Vol. 39, Issue 8, pp. 8225-30, (2012) (PubMed).

Murakami, Mizuno, Taniguchi, Fujii, Ishiguro, Fukui, Akatsuka, Horio, Hida, Kondo, Toyokuni, Osada, Sekido: "LATS2 is a tumor suppressor gene of malignant mesothelioma." in: Cancer research, Vol. 71, Issue 3, pp. 873-83, (2011) (PubMed).

Mardin, Lange, Baxter, Hardy, Scholz, Fry, Schiebel: "Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction." in: Nature cell biology, Vol. 12, Issue 12, pp. 1166-76, (2010) (PubMed).

Target Details for SAV1

Target: SAV1 (Salvador Homolog 1 (SAV1))

Alternative Name: SAV1

Background: Full Gene Name: salvador homolog 1 (Drosophila)
Synonyms: SAV,WW45,WWP4

Gene ID: 60485

NCBI Accession: NM_021818

Pathways: Stem Cell Maintenance