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Recombinant DDDDK Tag antibody

The Mouse Monoclonal anti-DDDDK Tag antibody has been validated for WB, ELISA and IF. It is suitable to detect DDDDK Tag in samples from .
Catalog No. ABIN5668122

Quick Overview for Recombinant DDDDK Tag antibody (ABIN5668122)

Target

DDDDK Tag

Antibody Type

Recombinant Antibody

Reactivity

Please inquire

Host

  • 9
  • 5
Mouse

Clonality

  • 11
  • 2
  • 1
Monoclonal

Conjugate

  • 13
  • 1
This DDDDK Tag antibody is un-conjugated

Application

  • 14
  • 9
  • 6
  • 6
  • 4
  • 3
  • 2
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone

M2-1
  • Purpose

    Anti-DDDDK-tag [M2.1], Mouse IgG1, kappa

    Specificity

    This antibody recognises the DYKDDDDK-tag, a widely used epitope tag.

    Characteristics

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    Purification

    Protein A affinity purified

    Immunogen

    Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.

    Isotype

    IgG1 kappa
  • Application Notes

    This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. This antibody's binding is not sensitive to calcium. This antibody recognises both native and denatured protein.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target

    DDDDK Tag

    Alternative Name

    DDDDK-tag

    Target Type

    Tag

    Background

    FLAG-tag, Equivalent to FLAG antibodies from Sigma, DYKDDDDK, DDDK, AspTyrLysAspAspAspAspLy
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