POU2F1 antibody

Catalog No. ABIN567788

The Mouse Monoclonal anti-POU2F1 antibody has been validated for WB, IP and EMSA. It is suitable to detect POU2F1 in samples from Human and Mouse.

Quick Overview for POU2F1 antibody (ABIN567788)

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This POU2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: YL15

Product Details anti-POU2F1 Antibody

Specificity: This antibody reacts with Oct-1 (90 kDa) on Western blotting. YL15 recognizes the POU-domain linker region of Human Oct-1.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant full-length Human Oct-1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for POU2F1

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Alternative Name: POU2F1 / OCT1

Background: Octamer transcrIPPtional factor 1 (Oct-1) is a 90 kDa transcrIPPtion factor that binds to the octamer motif ATTTGCAT. This ubiquitous regulatory element is most conspicuous in B cells, where it is found in every immunoglobulin (Ig) promoter and in most Ig enhancers. In addition, Oct-1 binding also activates the promoters of the genes for some small nuclear RNAs (snRNA) and for histone H2B. Oct-1 protein is expressed in multIPPle tissues although some Oct-1 isoforms appear to be tissue specific and the homologous Oct-2 appears to be restricted in expression to B-cells. At least in B-cells, the Oct proteins bind to DNA together with another protein transcrIPPtion factor, octamer binding factor-1 (OBF-1), to form a ternary complex.Synonyms: NF-A1, OTF1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, Otf-1, POU domain class 2 transcription factor 1

Gene ID: 5451

NCBI Accession: NP_002688

UniProt: P14859