Caspase 12 antibody (N-Term)

Catalog No. ABIN567795

This anti-Caspase 12 antibody is a Rat Monoclonal antibody detecting Caspase 12 in WB, IF, IP and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Caspase 12 antibody (N-Term) (ABIN567795)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 14F7

Product Details anti-Caspase 12 Antibody

Binding Specificity: AA 95-318, N-Term

Specificity: This antibody reacts with Caspase-12 (~53 kDa) on Western blotting, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.

Purification: Protein-G Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant Caspase-12 N-terminal fragment (aa 95-318).

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12

Background: Caspase-12 is localized to the endoplasmic reticulum (ER), but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis and accumulation of excess proteins in ER, but not by membrane- or mitochondrial-targeted apoptotic signals. One well-known cause of ER stress in neurons is the accretion of amyloid-beta, the insoluble protein that forms plaques in the brains of patients with Alzheimer's disease. Caspase-12 mediates cytotoxicity induced by amyloid-beta and ER stress-induced apoptosis. Caspase-12 is ubiquitously expressed in tissues. Similar to other members of the Caspase family, Caspase-12 requires cleavage of the prodomain to activate its proapoptotic form.Synonyms: CASP-12, CASP12, Inactive caspase-12

Gene ID: 100506742

NCBI Accession: NP_001177945

UniProt: Q6UXS9

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response