CD3 antibody

Catalog No. ABIN567797

The Hamster Monoclonal anti-CD3 antibody has been validated for IP and WB. It is suitable to detect CD3 in samples from Human.

Quick Overview for CD3 antibody (ABIN567797)

Target: CD3

Reactivity: Human

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Clone: HAM25-1352

Product Details anti-CD3 Antibody

Specificity: This antibody reacts with CD3 epsilon (23 kDa) on Western blotting.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Human T3 cells.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: The antigen receptor of the T lymphocyte consists of two variable T-cell receptor chains (either TCR-apha, TCR-beta or TCR-gamma, TCR-delta) noncovalently linked to four different invariant membrane proteins ((CD3-γ , CD3-δ , CD3-ε , and the CD3-ζ homodimer). The CD3 genes are expressed early in thymocyte development, preceding the rearrangement and expression of the T-cell receptor genes. The CD3 epsilon polypeptide contributes to the cell surface display as well as to the signal transduction properties of the T-cell antigen receptor complex. Intriguingly, the distribution of CD3 epsilon is not restricted to T cells, since activated mouse, human, and avian natural killer (NK) cells do express intracytoplasmic CD3 epsilon polypeptides. CD3 epsilon is also present in the cytoplasm of fetal thymic T/NK bipotential progenitor cells, suggesting that it constitutes a component of the NK differentiation program.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway