FH antibody (Biotin)

Catalog No. ABIN568201

This Rabbit Polyclonal antibody specifically detects FH in WB, IF, IP, EIA, ID, RIA and DB. It exhibits reactivity toward Pig.

Quick Overview for FH antibody (Biotin) (ABIN568201)

Target: FH (Fumarate Hydratase (FH))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FH antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)

Product Details anti-FH Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~7.6

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Fumarase is isolated and purified from Porcine heart Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: Fumarase

Background: Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812], also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia. Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids). Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].Synonyms: HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase

Gene ID: 9823

UniProt: P10173