Glucose-6-Phosphate Dehydrogenase antibody (Biotin)

Catalog No. ABIN568214

The Rabbit Polyclonal anti-Glucose-6-Phosphate Dehydrogenase antibody has been validated for WB, IF, IP, EIA, ID, RIA and DB. It is suitable to detect Glucose-6-Phosphate Dehydrogenase in samples from Saccharomyces cerevisiae.

Quick Overview for Glucose-6-Phosphate Dehydrogenase antibody (Biotin) (ABIN568214)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody (Biotin)

Specificity: This antibody recognizes Glucose-6-Phosphate Dehydrogenase from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Absorption / Emission: Biotin/IgG ~5.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Glucose-6-Phosphate Dehydrogenase isolated and purified from Baker’s Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD

Background: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-phosphate dehydrogenase, zwf

Gene ID: 855480

NCBI Accession: NP_014158

UniProt: P11412

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones