Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Glycerokinase isolated and purified from Bacillus stearothermophilus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
GK
Reactivity: Human
ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL of sterile distilled water
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Target
Glycerol Kinase (GK)
Alternative Name
Glycerol Kinase
Background
Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors, UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway.Synonyms: ATP:glycerol 3-phosphotransferase, GK, GKD, Glycerokinase