HADH antibody (Biotin)

Catalog No. ABIN568259

The Rabbit Polyclonal anti-HADH antibody has been validated for WB, IP, IF, DB, EIA, ID and RIA. It is suitable to detect HADH in samples from Pig.

Quick Overview for HADH antibody (Biotin) (ABIN568259)

Target: HADH (Hydroxyacyl-CoA Dehydrogenase (HADH))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HADH antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-HADH Antibody (Biotin)

Specificity: Beta-Hydroxyacyl-CoA Dehydrogenase from Porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~6.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: beta-Hydroxyacyl-CoA Dehydrogenase isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for HADH

Target: HADH (Hydroxyacyl-CoA Dehydrogenase (HADH))

Alternative Name: HADH / HCDH

Background: Synonyms: HAD, HADHSC, Hydroxyacyl-coenzyme A dehydrogenase mitochondrial, Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase, SCHAD, Short chain 3-hydroxyacyl-CoA dehydrogenase

Gene ID: 397604

NCBI Accession: NP_999496

UniProt: P00348

Pathways: Negative Regulation of Hormone Secretion, Monocarboxylic Acid Catabolic Process