MDH1 antibody

Catalog No. ABIN568316

The Rabbit Polyclonal anti-MDH1 antibody has been validated for WB, IP, IF, EIA, ID, DB and RIA. It is suitable to detect MDH1 in samples from Pig.

Quick Overview for MDH1 antibody (ABIN568316)

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Product Details anti-MDH1 Antibody

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Malic dehydrogenase is isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDHA

Background: Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic

Gene ID: 396894

NCBI Accession: NP_999039

UniProt: P11708