Pepsin antibody (Biotin)

Catalog No. ABIN568352

The Rabbit Polyclonal anti-Pepsin antibody has been validated for WB, IP, EIA, IF, ID, DB and RIA. It is suitable to detect Pepsin in samples from Pig.

Quick Overview for Pepsin antibody (Biotin) (ABIN568352)

Target: Pepsin

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pepsin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB), Radioimmunoassay (RIA)

Product Details anti-Pepsin Antibody (Biotin)

Specificity: Pepsin from Porcine stomach mucosa. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~ 4.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Pepsin isolated and purified from Porcine stomach mucosa. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Pepsin

Target: Pepsin

Background: Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I

Gene ID: 396892

NCBI Accession: NP_999038

UniProt: P00791