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Alkaline Phosphatase antibody

This anti-Alkaline Phosphatase antibody is a Rabbit Polyclonal antibody detecting Alkaline Phosphatase in WB, IF, IP, EIA, ID, RIA and DB. Suitable for E. coli.
Catalog No. ABIN568362

Quick Overview for Alkaline Phosphatase antibody (ABIN568362)

Target

See all Alkaline Phosphatase (ALP) Antibodies
Alkaline Phosphatase (ALP)

Reactivity

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E. coli

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Alkaline Phosphatase antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)
  • Specificity

    Alkaline Phosphatase from Escherichia coli. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Purification

    Ammonium Sulphate Precipitation and Ion Exchange Chromatography

    Immunogen

    Alkaline Phosphatase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore by adding 1.0 mL of sterile distilled water

    Concentration

    10.0 mg/mL

    Buffer

    PBS, pH 7.2 without preservatives and foreign proteins

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • Target

    Alkaline Phosphatase (ALP)

    Alternative Name

    Alkaline Phosphatase

    Background

    Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: JW0374, b0383, phoA

    Gene ID

    945041

    NCBI Accession

    AP_001034

    UniProt

    P00634
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